tailieunhanh - Báo cáo y học: " Modulation of expression and cellular distribution of p21 by macrophage migration inhibitory factor"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Modulation of expression and cellular distribution of p21 by macrophage migration inhibitory factor. | Journal of Inflammation BioMed Central Research Modulation of expression and cellular distribution of p21 by macrophage migration inhibitory factor Elliott Taranto Jin RXue Eric F Morand and Michelle Leech Address Centre for Inflammatory Diseases Monash University Department of Medicine Monash Medical Centre Clayton Melbourne Australia Email Elliott Taranto - taranto@ Jin RXue - jinxue@ Eric F Morand - Michelle Leech - Corresponding author Open Access Published 24 August 2009 Received I April 2009 Journal of Inflammation 2009 6 24 doi 1476-9255-6-24 Accepted 24 August 2009 This article is available from http content 6 1 24 2009 Taranto et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background The pleiotropic protein MIF macrophage migration inhibitory factor has been demonstrated to modulate several key proteins governing cell cycle control and is considered to contribute to cell growth and differentiation. In this study we investigated the effect of MIF on the expression and cellular distribution of the CDK inhibitor p21. Methods The effect of endogenous MIF on p21 expression and distribution was examined by comparing murine dermal fibroblasts derived from wt and MIF - - mice. The effect of MIF on cell growth and apoptotic rates was compared using 3H-Thymidine incorporation assays and annexin V PI assays respectively. Total p21 protein levels were compared using flow cytometry and western blotting. p21 mRNA was assessed by RT-PCR. Intracellular p21 staining was performed to assess cellular .