tailieunhanh - Báo cáo y học: "P38 MAP kinase inhibitors as potential therapeutics for the treatment of joint degeneration and pain associated with osteoarthritis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: P38 MAP kinase inhibitors as potential therapeutics for the treatment of joint degeneration and pain associated with osteoarthritis. | Journal of Inflammation BioMed Central Open Access P38 MAP kinase inhibitors as potential therapeutics for the treatment of joint degeneration and pain associated with osteoarthritis Kimberly K Brown Sandra A Heitmeyer Erin B Hookfin Lily Hsieh Maria Buchalova Yetunde O Taiwo and Michael J Janusz Address Procter Gamble Pharmaceuticals Inc. 8700 Mason-Montgomery Rd. Mason OH 45040-9462 USA Email Kimberly K Brown - brown2kk@ Sandra A Heitmeyer - Erin B Hookfin - Lily Hsieh - peacelily2000@ Maria Buchalova - Yetunde O Taiwo - taiwoye@ Michael J Janusz - Corresponding author Published 4 December 2008 Received 12 February 2008 Accepted 4 December 2008 Journal of Inflammation 2008 5 22 doi l476-9255-5-22 This article is available from http content 5 1 22 2008 Brown et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Evaluate the potential role of p38 inhibitors for the treatment of osteoarthritis using an animal model of joint degeneration iodoacetate-induced arthritis and a pain model Hargraeves assay . Methods P38 kinase activity was evaluated in a kinase assay by measuring the amount of phosphorylated substrate ATF2 using a phosphoATF2 Thr71 specific primary antibody and an alkaline phosphate coupled secondary antibody and measuring the OD at 405 nm. TNFa and IL-1P secretion from LPS stimulated THP-1 monocytic cells and human peripheral blood mononuclear cells were measured by ELISA. Rats treated with vehicle or p38 inhibitor were injected intra-articularly in one knee with iodoacetate and damage to the tibial plateau was assessed from digitized images captured .

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