tailieunhanh - Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophages. | Journal of Inflammation BioMed Central Research Open Access Heat shock protein and heat shock factor I expression and localization in vaccinia virus infected human monocyte derived macrophages Aleksandra Kowalczyk Krzysztof Guzik Kinga Slezak Jakub Dziedzic and Hanna Rokita Address Jagiellonian University Faculty of Biotechnology 7 Gronostajowa St. 30-387 Krakow Poland Email Aleksandra Kowalczyk - kowalczyk@ Krzysztof Guzik - chris@ Kinga Slezak - kingas02@ Jakub Dziedzic - jakub-Dziedzic@ Hanna Rokita - hannar@ Corresponding author Published 24 October 2005 Received 29 April 2005 Journal of Inflammation 2005 2 12 doi 1476-9255-2-12 Accepted 24 October 2005 This article is available from http content 2 1 12 2005 Kowalczyk et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Viruses remain one of the inducers of the stress response in the infected cells. Heat shock response induced by vaccinia virus VV infection was studied in vitro in human blood monocyte derived macrophages MDMs as blood cells usually constitute the primary site of the infection. Methods Human blood monocytes were cultured for 12 - 14 days. The transcripts of heat shock factor 1 HSF1 heat shock protein 70 HSP70 heat shock protein 90 HSP90 and two viral genes E3L and F17R were assayed by reverse transcriptase-polymerase chain reaction RT-PCR and the corresponding proteins measured by Western blot. Heat shock factor 1 DNA binding activities were estimated by electrophoretic mobility shift assay EMSA and its subcellular localization analyzed by immunocytofluorescence. Results It appeared that infection with vaccinia virus leads to .