tailieunhanh - Báo cáo y học: "Product inhibition of secreted phospholipase A2 may explain lysophosphatidylcholines' unexpected therapeutic properties"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Product inhibition of secreted phospholipase A2 may explain lysophosphatidylcholines' unexpected therapeutic properties. | Journal of Inflammation BioMed Central Research Product inhibition of secreted phospholipase A2 may explain lysophosphatidylcholines unexpected therapeutic properties Timothy J Cunningham Lihua Yao and Angel Lucena Address Neurobiology and Anatomy Drexel University College of Medicine Philadelphia PA 19129 USA Email Timothy J Cunningham - tcunning@ Lihua Yao - lihuayao@ Angel Lucena - all26@ Corresponding author Open Access Published 22 October 2008 Received 20 June 2008 _AAAO J- _IA I in 1 -7 Airr r 1-7 Accepted 22 October 2008 Journal of Inflammation 2008 5 17 doi 1476-9255-5-17 This article is available from http content 5 1 17 2008 Cunningham et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Lysophosphatidylcholines lysoPCs are products of phospholipase A2 PLA2 enzyme activity and like the enzyme have a direct role in toxic inflammatory responses in variety of organ systems. Paradoxically reduced plasma lysoPC levels have been noted in sepsis patients and systemic treatment with lysoPCs is therapeutic in rodent models of sepsis and ischemia. These observations suggest that elevation of plasma levels of these lipids can actually help to relieve serious inflammatory conditions. We demonstrate that specific lysoPCs act as uncompetitive product inhibitors of plasma secreted PLA2 enzymes sPLA2s especially under conditions of elevated enzyme activity thus providing a feedback mechanism for the observed anti-inflammatory effects of these compounds. Methods Thin layer chromatography and mass spectroscopy were used to estimate total lysoPC concentration and the relative contributions of different lysoPC species in .

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