tailieunhanh - Báo cáo y học: "Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro. | Journal of Inflammation BioMed Central Research Anti-inflammatory effects of ciprofloxacin in s. aureus Newman induced nasal inflammation in vitro F Sachse 1 C von Eiff2 K Becker2 and C Rudack1 Open Access Address Department of Otorhinolaryngology Head and Neck Surgery University of Muenster Germany and 2Department of Microbiology University of Muenster Germany Email F Sachse - sachsef@ C von Eiff- eiffc@ K Becker-kbecker@ C Rudack - rudackc@ Corresponding author Published 29 July 2008 Received 21 February 2008 Journal of Inflammation 2008 5 11 doi 1476-9255-5-11 Accepted 29 July 2008 This article is available from http content 5 1 1 1 2008 Sachse et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Objectives Chronic rhinosinusitis CRS is a chronic inflammatory disease of the nasal mucosa. Recent studies suggest that s. aureus enterotoxins may play an etiologic role in the development of CRS. Apart from surgery and repeated courses of steroids macrolide antibiotics have been reported to exert anti-inflammatory effects in CRS. Similar effects have been reported for fluoroquinolones on various cell types. Since these effects have poorly been characterized in CRS we examined anti-inflammatory effects of ciprofloxacin on human nasal epithelial cells HNECs . Methods Inflammation was induced in HNECs cultured from nasal turbinate mucosa with supernatants of s. aureus Newman for 12 hours. Subsequently HNECs were coincubated with s. aureus Newman and ciprofloxacin X 10-5 M clarithromycin 10-6 M or prednisolone 10-5 M for another 12 hours. IL-8 synthesis was quantified after 12 and 24 hours by ELISA. Results .

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