tailieunhanh - Báo cáo y học: " C-reactive protein does not opsonize early apoptotic human neutrophils, but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophages"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: C-reactive protein does not opsonize early apoptotic human neutrophils, but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophages. | Journal of Inflammation BioMed Central Research Open Access C-reactive protein does not opsonize early apoptotic human neutrophils but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophages Simon P Hart Karen M Alexander Shonna M MacCall and Ian Dransfield Address MRC Centre for Inflammation Research University of Edinburgh Medical School Teviot Place Edinburgh EH8 9AG UK Email Simon P Hart - Karen M Alexander - karenmalexander@ Shonna M MacCall - Ian Dransfield - Corresponding author Published 31 May 2005 Received 25 October 2004 Journal of Inflammation 2005 2 5 doi l476-9255-2-5 Accepted 31 May 2005 This article is available from http content 2 1 5 2005 Hart et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background It has been reported that C-reactive protein CRP binds both leukocyte FcyRIIA CD32 and the plasma membrane of apoptotic cells. Since FcyRIIA becomes functionally enabled during neutrophil apoptosis we sought to determine whether CRP bound to apoptotic neutrophils via FcyRIIA. Methods We prepared directly labelled CRP and demonstrated that it was essentially free of IgG. We looked for evidence of CRP binding to intact membrane impermeable apoptotic human neutrophils and to FcYRIIA-transfected Jurkat cells. We examined the functional consequences of incubation with CRP upon phagocytosis of apoptotic cells by human monocyte-derived macrophages. Results We could not detect binding of purified soluble CRP to classical early apoptotic human neutrophils or to FcYRIIA-transfected Jurkat cells. In contrast membrane-permeable late apoptotic neutrophils