tailieunhanh - Báo cáo y học: "The tripeptide feG inhibits leukocyte adhesion"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The tripeptide feG inhibits leukocyte adhesion. | Journal of Inflammation BioMed Central Research The tripeptide feG inhibits leukocyte adhesion Ronald D Mathison Emily Christie and Joseph S Davison Open Access Address University of Calgary Faculty of Medicine Department of Physiology and Biophysics 3330 Hospital Drive NW Calgary Alberta T2N 4N1 Canada Email Ronald D Mathison - rmathiso@ Emily Christie - Joseph S Davison - jdavison@ Corresponding author Published 20 May 2008 Received 12 September 2007 Journal of Inflammation 2008 5 6 doi 186 1476-9255-5-6 Accepted 20 May 2008 This article is available from http content 5 1 6 2008 Mathison et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background The tripeptide feG D-Phe-D-Glu-Gly is a potent anti-inflammatory peptide that reduces the severity of type I immediate hypersensitivity reactions and inhibits neutrophil chemotaxis and adhesion to tissues. feG also reduces the expression of p1-integrin on circulating neutrophils but the counter ligands involved in the anti-adhesive actions of the peptide are not known. In this study the effects of feG on the adhesion of rat peritoneal leukocytes and extravasated neutrophils to several different integrin selective substrates were evaluated. Results The adhesion of peritoneal leukocytes and extravasated neutrophils from rats to adhesive proteins coated to 96-well plates was dependent upon magnesium Mg2 ion suggestive of integrin-mediated adhesion. feG inhibited leukocyte adhesion but only if the cells were stimulated with PAF 10-9M indicating that feG s actions in vitro require cell activation. In the dose .