tailieunhanh - Báo cáo hóa học: " Evaluating interaction forces between BSA and rabbit anti-BSA in sulphathiazole sodium, tylosin and levofloxacin solution by AFM"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí hóa hoc quốc tế đề tài : Evaluating interaction forces between BSA and rabbit anti-BSA in sulphathiazole sodium, tylosin and levofloxacin solution by AFM | Wang et al. Nanoscale Research Letters 2011 6 579 http content 6 1 579 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Evaluating interaction forces between BSA and rabbit anti-BSA in sulphathiazole sodium tylosin and levofloxacin solution by AFM Congzhou Wang1 2 Jianhua Wang1 2 and Linhong Deng1 2 Abstract Protein-protein interactions play crucial roles in numerous biological processes. However it is still challenging to evaluate the protein-protein interactions such as antigen and antibody in the presence of drug molecules in physiological liquid. In this study the interaction between bovine serum albumin BSA and rabbit anti-BSA was investigated using atomic force microscopy AFM in the presence of various antimicrobial drugs sulphathiazole sodium tylosin and levofloxacin under physiological condition. The results show that increasing the concentration of tylosin decreased the single-molecule-specific force between BSA and rabbit anti-BSA. As for sulphathiazole sodium it dramatically decreased the specific force at a certain critical concentration but increased the nonspecific force as its concentration increasing. In addition the presence of levofloxacin did not greatly influence either the specific or nonspecific force. Collectively these results suggest that these three drugs may adopt different mechanisms to affect the interaction force between BSA and rabbit anti-BSA. These findings may enhance our understanding of antigen antibody binding processes in the presence of drug molecules and hence indicate that AFM could be helpful in the design and screening of drugs-modulating protein-protein interaction processes. 1. Introduction A molecular level understanding of protein-protein interactions is fundamentally important in the life sciences. A number of human diseases are closely related to the protein-protein association or dissociation events and thus probing and characterizing these interactions have .

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