tailieunhanh - Báo cáo y học: "Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet. | Journal of Inflammation BioMed Central Research Open Access Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet Hong Huang1 3 Tongzheng Liu1 Jane L Rose1 Rachel L Stevens1 and Dale G Hoyt 1 2 Address 1Division of Pharmacology The Ohio State University College of Pharmacy Columbus OH 43210 USA 2The Dorothy M. Davis Heart and Lung Research Institute Columbus OH 43210 USA and 3Center for Cardiovascular Medicine Columbus Children s Research Institute Columbus OH 43205 USA Email Hong Huang - huangh@ Tongzheng Liu - Jane L Rose - Rachel L Stevens - Dale G Hoyt - Corresponding author Published 12 November 2007 Received 19 June 2007 Accepted 12 November 2007 Journal of Inflammation 2007 4 22 doi 1476-9255-4-22 This article is available from http content 4 1 22 2007 Huang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background It was hypothesized that a pro-atherogenic high saturated fat and cholesterol diet HCD would increase the inflammatory response to E. coli endotoxin LPS and increase its concentration in plasma after administration to mice. Methods C57Bl 6 mice were fed a HCD or a control diet CD for 4 weeks and then treated with saline 1 or 2 mg LPS kg ip. Liver injury alanine 2-oxoglutarate aminotransferase and aspartate aminotransferase collagen staining circulating cytokines tumor necrosis factor-a interleukin-6 and interferon-Y factors that can bind LPS serum amyloid A apolipoprotein A1 LPS binding protein and CD14 and plasma levels of LPS were measured. The hepatic response was assessed by measuring vascular cell adhesion molecule VCAM -1 .

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