tailieunhanh - Báo cáo y học: "Immunization with genetically attenuated P52deficient Plasmodium berghei sporozoites induces a long-lasting"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Immunization with genetically attenuated P52deficient Plasmodium berghei sporozoites induces a long-lasting . | Douradinha et al. Journal of Immune Based Therapies and Vaccines 2011 9 6 http content 9 1 6 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES SHORT REPORT Open Access Immunization with genetically attenuated P52-deficient Plasmodium berghei sporozoites induces a long-lasting effector memory CD8 T cell response in the liver Rri inn ITmi imd im In n 1 2 i inIicell mn nilL3 8 iTciCi et- I nm .in l omnrt4 H n Hini l l 1 brie I linen3 Diuno Douiadiiuia ivielissa van Dijk Geeitjan van Jdiieii Shahid M Khan Ciiiis J janse Ănrlv D lA ntd rc3 9 PoHonr A Cai nr . . sin4 Arinin IO IP I I itv4 Rri nn jl . i- intnr3 3 l lnrln l l i lotn - 2 nnrl Andy P waters Robeit w Saueiwein Adrian JF Luty Diuno Silva Santos Maria M Mota and Sabrina Epiphanio1 2 6 Abstract Background The induction of sterile immunity and long lasting protection against malaria has been effectively achieved by immunization with sporozoites attenuated by gamma-irradiation oi through deletion of genes. Foi mice immunized with radiation attenuated sporozoites RAS it has been shown that intiahepatic effectoi memoiy CD8 T cells aie critical foi protection. Recent studies have shown that immunization with genetically attenuated parasites GAP in mice is also confeiied by livei effectoi memoiy CD8 T cells. Findings In this study we analysed effectoi memoiy cell responses aftei immunization of GAP that lack the P52 piotein. We demonstrate that immunization with p52-GAP sporozoites also results in a strong inciease of effectoi memoiy CD8 T cells even 6 months aftei immunization whereas no specific CD4 effectoi T cells response could be detected. In addition we show that the inciease of effectoi memoiy CD8 T cells is specific foi the livei and not foi the spleen oi lymph nodes. Conclusions These results indicate that immunization of mice with P. berghei p52-GAP results in immune responses that aie comparable to those induced by RAS oi GAP lacking expression of UIS3 oi UIS4 with an important .