tailieunhanh - Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment. | Roeska and Seilheimer Journal of Immune Based Therapies and Vaccines 2010 8 6 http content 8 1 6 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES ORIGINAL RESEARCH Open Access Antiviral activity of Engystol and Gripp-Heel an in-vitro assessment Kerstin Roeska Bernd Seilheimer Abstract Background Infections with respiratory viruses can activate the innate immune response - an important host defence mechanism in the early stage of viral infection. Interferon IFN release triggered by virus infection is an important factor in establishing an antiviral state where IFN activation occurs prior to the onset of the adaptive immune response. The two ultra-low-dose combination medications Engystol and Gripp-Heel have documented efficacy for the treatment of the respiratory infections. However the underlying antiviral mechanisms remain elusive. Methods It was the goal to investigate whether Engystol and Gripp-Heel display antiviral activity in a prophylactic treatment protocol 2 24 and 48 h pre-incubation using a plaque reduction assay and whether the medications affect the release of type 1 IFN in virus-susceptible cell lines and human peripheral blood mononuclear cells PBMCs . Results Both medications demonstrate prophylactic effect against viral respiratory virus replication. However when the incubation was continued for up to 5 days both medications exhibited a pronounced antiviral effect which was dependent on the pre-incubation time. Moreover in co-stimulated HeLa cells as well as in activated PBMCs Gripp-Heel and Engystol demonstrated an increased type 1 IFN production. Conclusions Engystol and Gripp-Heel inhibited the replication of a variety of respiratory viruses. Additionally we showed that pre-incubation affects the magnitude of the inhibitory effect differently for the various tested viruses. Both medications stimulate type 1 IFN release in different cell systems which suggests that their antiviral activity may be mediated possibly via .

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