tailieunhanh - Báo cáo y học: " Probing local innate immune responses after mucosal immunisation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Probing local innate immune responses after mucosal immunisation. | Hall et al. Journal of Immune Based Therapies and Vaccines 2010 8 5 http content 8 1 5 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES ORIGINAL RESEARCH Open Access Probing local innate immune responses after mucosal immunisation Lindsay J Hall Simon Clare Gordon Dougan Abstract Background Intranasal immunisation is potentially a very effective route for inducing both mucosal and systemic immunity to an infectious agent. Methods Balb c mice were intranasally immunised with the mucosal adjuvant heat labile toxin and the Mycobacterium tuberculosis fusion protein Ag85B-ESAT6 and early changes in innate immune responses within local mucosal tissues were examined using flow cytometry and confocal microscopy. Antigen-specific humoral and cellular immune responses were also evaluated. Results Intranasal immunisation induced significant changes in both number and distribution of dendritic cells macrophages and neutrophils within the nasal-associated lymphoid tissue and cervical lymph nodes in comparison to controls as early as 5 h post immunisation. Immunisation also resulted in a rapid and transient increase in activation marker expression first in the nasal-associated lymphoid tissue and then in the cervical lymph nodes. This heightened activation status was also apparent from the pro-inflammatory cytokine profiles of these innate populations. In addition we also showed increased expression and distribution of a number of different cell adhesion molecules early after intranasal immunisation within these lymphoid tissues. These observed early changes correlated with the induction of a TH1 type immune response. Conclusions These data provide insights into the complex nature of innate immune responses induced following intranasal immunisation within the upper respiratory tract and may help clarify the concepts and provide the tools that are needed to exploit the full potential of mucosal vaccines. Background In recent years the nasal route for .