tailieunhanh - Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array. | Journal of Inflammation BioMed Central Open Access Detection of cytokine protein expression in mouse lung homogenates using suspension bead array Eric McDuffie 1 Leslie Obert12 Jonathan Chupka11 and Robert Sigler1 Address 1Drug Safety Evaluation Department Esperion a Division of Pfizer Research and Development Ann Arbor Michigan 48108 USA and 2Worldwide Safety Sciences Department Pathology Section Pfizer Research and Development Ann Arbor Michigan 48105 USA Email Eric McDuffie - Leslie Obert - Jonathan Chupka - Robert Sigler - Corresponding author tEqual contributors Published 29 November 2006 Received 05 May 2006 Accepted 29 November 2006 Journal of Inflammation 2006 3 15 doi 1476-9255-3-15 This article is available from http content 3 1 15 2006 McDuffie et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The objective for this present study was to determine whether or not suspension bead array is a feasible method to detect changes in cytokine protein expression in mouse lung tissue homogenates. Here we report on suspension bead array as a feasible method for detection of lipopolysaccharide LPS -evoked changes in cytokine protein expression in mouse lung tissue homogenates. Materials and methods Mice were treated ml intraperitoneal . with phosphate buffered saline PBS or LPS mg ml and sacrificed at either 2- or 24-hours post treatment. Formalin-fixed and paraffin-embedded lung sections were evaluated by light microscopy. Flash frozen lung tissues were homogenized for measurement of various cytokine protein expression levels using suspension bead array .

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