tailieunhanh - Báo cáo y học: "Early Migrating leukocytes are the source of Peroxiredoxin V during inflammation in the airway"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Migrating leukocytes are the source of Peroxiredoxin V during inflammation in the airways. | Journal of Inflammation BioMed Central Research Open Access Migrating leukocytes are the source of Peroxiredoxin V during inflammation in the airways Raisa I Krutilina3 Andrei V Kropotov1 3 Christian Leutenegger2 3 and Vladimir B Serikov 3 Address 1Institute of Cytology Russian Academy of Sciences St. Petersburg 194021 Russia 2University of California Davis Davis CA 95616 USA and 3Children s Hospital Oakland Research Institute Oakland CA 94609 USA Email Raisa I Krutilina - rakrutilina@ Andrei V Kropotov - akropotov@ Christian Leutenegger - cmleutenegger@ Vladimir B Serikov - vserikov@ Corresponding author Published 04 October 2006 Received 29 March 2006 Accepted 04 October 2006 Journal of Inflammation 2006 3 13 doi 1476-9255-3-13 This article is available from http content 3 1 13 2006 Krutilina et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We characterized changes in expression of the antioxidant protein Peroxiredoxin V PRXV during airway inflammation. Methods Studies in anesthetized rats and mice PRXV expression determined by Western blot analyses and immunohistochemistry PRXV m-RNA expression determined by Taq-Man RT-PCR. Results Bacterial lung inflammation did not change expression of PRXV in murine epithelia but produced massive influx of leukocytes highly expressing PRXV. Endotoxin and f-MLP induced leukocyte migration in rat trachea but did not change mRNA levels and PRXV protein expression in tracheal epithelial cells. In primary airway cell culture cow alveolar epithelial cells A549 or coculture of A549 with murine macrophages exposure to live bacteria increased expression of PRXV which required serum. .