tailieunhanh - Báo cáo y học: " PCEP enhances IgA mucosal immune responses in mice following different immunization routes with influenza virus antigens"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: PCEP enhances IgA mucosal immune responses in mice following different immunization routes with influenza virus antigens. | Eng et al. Journal of Immune Based Therapies and Vaccines 2010 8 4 http content 8 1 4 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES ORIGINAL RESEARCH Open Access PCEP enhances IgA mucosal immune responses in mice following different immunization routes with influenza virus antigens 1 f 1 f 1 2 1 Nelson F Eng Srinivas Garlapati Volker Gerdts Lorne A Babiuk George K Mutwiri Abstract Background We previously demonstrated that polyphosphazenes particularly PCEP enhance immune responses in mice immunized subcutaneously and intranasally. The objective of the present study was to investigate the efficacy of polyphosphazenes as adjuvants when delivered through different routes of vaccine administration. Methods BALB c mice were immunized through intranasal subcutaneous oral and intrarectal delivery with vaccine formulations containing either influenza X 31 antigen alone or formulated in PCEP. Serum and mucosal washes were collected and assayed for antigen-specific antibody responses by ELISA while splenocytes were assayed for antigen-specific cytokine production by ELISPOT. Results Intranasal immunization with PCEP X 31 induced significantly higher IgA titers in all mucosal secretions lung nasal and vaginal compared to the other routes. Serum analysis showed that all mice given the PCEP X 31 combination showed evidence of enhanced IgG2a titers in all administered routes indicating that PCEP can be effective as an adjuvant in enhancing systemic immune responses when delivered via different routes of administration. Conclusions We conclude that PCEP is a potent and versatile mucosal adjuvant that can be administered in a variety of routes and effectively enhances systemic and local immune responses. Furthermore intranasal immunization was found to be the best administration route for enhancing IgA titers providing further evidence for the potential of PCEP as a mucosal adjuvant. Background The high costs associated with the treatment of infectious .