tailieunhanh - Báo cáo y học: "Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers."

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers. | Hughes et al. Journal of Inflammation 2010 7 2 http content 7 1 2 JOURNAL OF INFLAMMATION RESEARCH Open Access Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers Stephen F Hughes1 6 Beverly D Hendricks2 David R Edwards3 Kirsty M Maclean4 Salah S Bastawrous5 Jim F Middleton6 Abstract Background It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement THR and total knee replacement TKR orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively. Methods During this pilot study ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b Mac-1 and CD62L cell surface expression intracellular production of H2O2 and elastase were measured as markers of leukocyte function. Von Willebrand factor vWF and soluble intercellular adhesion molecule-1 sICAM-1 were measured as markers of endothelial activation. Results The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers suggestive of increased inflammatory reactions postoperatively. Specifically THR and TKR surgery resulted in a leukocytosis this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H2O2 production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of .