tailieunhanh - Báo cáo y học: "Thrombin increases inflammatory cytokine and angiogenic growth factor secretion in human adipose cells in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Thrombin increases inflammatory cytokine and angiogenic growth factor secretion in human adipose cells in vitro. | Journal of Inflammation BioMed Central Research Thrombin increases inflammatory cytokine and angiogenic growth factor secretion in human adipose cells in vitro Jennifer L Strande 1 and Shane A Phillips1 2 Open Access Address Division of Cardiovascular Medicine Medical College of Wisconsin Milwaukee WI USA and 2Department of Physical Therapy University of Illinois Chicago IL USA Email Jennifer L Strande - jstrande@ Shane A Phillips - shanep@ Corresponding author Published 6 March 2009 Received 13 October 2008 Accepted 6 March 2009 Journal of Inflammation 2009 6 4 doi l476-9255-6-4 This article is available from http content 6 1 4 2009 Strande and Phillips licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Abdominal obesity is associated with pro-thrombotic and inflammatory states. Therefore the purpose of this study was to examine the expression of thrombin receptors PAR1 and PAR4 human adipose tissue and whether thrombin stimulates an inflammatory cytokine and growth factor profile in human adipose tissue. Methods Human adipose tissue isolated preadipocytes and differentiated adipocytes were used in this study. PAR1 and PAR4 mRNA and protein were detected by RT-PCR and immunoblot analysis in both adipose tissue and adipose microvessels. In separate studies IL-1P IL-6 MCP-1 TNF-a IL-10 FGF-2 VEGF and PDGF production were measured from adipose tissue n 5 adipocytes n 5 and preadipocytes n 3 supernatants with and without thrombin 1 or 10 U ml 24 hrs treatment. Results Thrombin increased cytokine secretion of IL-1P IL-6 MCP-1 and TNF-a and growth factor secretion of VEGF from adipocytes along with MCP-1 and VEGF from preadipocytes. The direct thrombin inhibitor