tailieunhanh - Báo cáo y học: " tế ngành y học dành cho các bạn tham khảo đề tài: Early Oxidative stress increases Fas ligand expression in endothelial cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Oxidative stress increases Fas ligand expression in endothelial cells. | Journal of Inflammation BioMed Central Research Open Access Oxidative stress increases Fas ligand expression in endothelial cells Mayumi Suzuki Kazutetsu Aoshiba and Atsushi Nagai Address First Department of Medicine Tokyo Women s Medical University 8-1 Kawada-cho Shinjuku-ku Tokyo 162-8666 Japan Email Mayumi Suzuki - kaoshiba@ Kazutetsu Aoshiba - kaoshiba@ AtsushiNagai - kaoshiba@ Corresponding author Published 19 July 2006 Received 23 January 2006 Journal of Inflammation 2006 3 11 doi 1476-9255-3-11 Accepted 19 July 2006 This article is available from http content 3 1 1 1 2006 Suzuki et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Fas ligand FasL induces apoptosis in Fas-bearing target cells such as leukocytes and up-regulation of FasL expression on the endothelium may contribute to anti-inflammatory reactions that attenuate leukocyte extravasation during inflammation. Since oxidants generated during inflammation and cigarette smoking may modulate endothelial function we examined the effect of H2O2 and cigarette smoke on endothelial FasL expression. Methods Human umbilical vein endothelial cells HUVECs were exposed to nontoxic concentrations of H2O2 and cigarette smoke extracts CSE . Membrane FasL expression was assessed by immunostaining with anti-FasL antibody followed by either monolayer-cell-based spectrofluorimetry or flow cytometry. Soluble FasL in culture supernatants was measured by enzyme-linked immunosorbent assay. For the cytotoxic assay HUVECs were exposed to H2O2 and co-cultured with neutrophils. Neutrophils were stained by a peroxidase diaminobenzidine-based reaction and apoptosis was evaluated on the .