tailieunhanh - Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro. | Journal of Inflammation BioMed Central Research Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro Lindsay Ramage Amanda C Jones and Clifford J Whelan Open Access Address School of Life Sciences University of Hertfordshire College Lane Hatfield Herts. AL10 9AB UK Email Lindsay Ramage - lindsayramage@ Amanda C Jones - Clifford J Whelan - wdrcjw@ Corresponding author Published 21 March 2006 Received 14 October 2005 Accepted 21 March 2006 Journal of Inflammation 2006 3 3 doi l476-9255-3-3 This article is available from http content 3 1 3 2006 Ramage et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background This study has investigated the ability of tobacco smoke and ingredients of tobacco smoke to induce apoptosis in the airway epithelial cell line A549. Method A549 cells were treated with 80 g ml Tobacco smoke condensate TSC 10 mM Nicotine 10 1M paraldehyde 10 1M hydrogen peroxide 1 1M Taxol Paclitaxel 100 50 and 25 cigarette smoke extract CSE . Following 4-48 h incubation apoptosis was measured morphologically following staining of cells with DAPI. TUNEL staining was also used to assess DNA damage after 24 and 48 h incubation. In addition loss of mitochondrial cytochrome C and activation of Bax-a early events in the apoptotic process were measured after 4 h of incubation. Results Incubation of A549 cells with vehicle Taxol TSC nicotine paraldehyde hydrogen peroxide and CSE caused a time-dependent detachment of the cells from the flask between 6 and 48 h. DAPI staining revealed that the cells remaining adhered to the flask appeared healthy whereas some of those that had detached .