tailieunhanh - Báo cáo khoa hoc:" Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210 | Journal of Negative Results in BioMedicine BioMed Central Research Open Access Despite WTI binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210 WTI does not influence expression of GP210 Magnus Olsson1 Milton A English2 Jacqueline Mason2 Jonathan D Licht2 and Peter Ekblom 1 Address department of Cell and Molecular Biology Section for Cell and Developmental Biology Lund University Sweden and 2Mount Sinai School of Medicine 1425 Madison Avenue New York NY 10029 USA Email Magnus Olsson - Milton A English - Jacqueline Mason - Jonathan D Licht - Peter Ekblom - Corresponding author Published 21 December 2004 Received 05 July 2004 Accepted 21 December 2004 Journal of Negative Results in BioMedicine 2004 3 7 doi 1477-5751-3-7 r This article is available from http content 3 1 7 2004 Olsson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Glycoprotein 210 GP210 is a transmembrane component of the nuclear pore complex of metazoans with a short carboxyterminus protruding towards the cytoplasm. Its function is unknown but it is considered to be a major structural component of metazoan nuclear pores. Yet our previous findings showed pronounced differences in expression levels in embryonic mouse tissues and cell lines. In order to identify factors regulating GP210 the genomic organization of human GP210 was analyzed in silico. Results The human gene was mapped to chromosome 3 and consists of 40 exons spread over 102 kb. The deduced 1887 amino acid showed a high degree of alignment homology to previously reported orthologues. Experimentally