tailieunhanh - Báo cáo y học: "Evaluation of recombinant invasive, non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen, Brucella"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evaluation of recombinant invasive, non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen, Brucella. | Journal of Immune Based Therapies and Vaccines BioMed Central Open Access Original research Evaluation of recombinant invasive non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen Brucella Jerome S Harms Marina A Durward Diogo M Magnani and Gary A Splitter Address Department of Pathobiological Sciences University of Wisconsin-Madison 1656 Linden Drive Madison WI 53706 USA Email Jerome S Harms - harms@ Marina A Durward - durward@ Diogo M Magnani - magnani@ Gary A Splitter - splitter@ Corresponding author Published 6 January 2009 Received 17 September 2008 Journal of Immune Based Therapies and Vaccines 2009 7 1 doi I86 I476-85I8-7-I Accepted 6 January 2009 This article is available from http content 7 1 1 2009 Harms et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background There is no safe effective human vaccine against brucellosis. Live attenuated Brucella strains are widely used to vaccinate animals. However these live Brucella vaccines can cause disease and are unsafe for humans. Killed Brucella or subunit vaccines are not effective in eliciting long term protection. In this study we evaluate an approach using a live non-pathogenic bacteria E. coli genetically engineered to mimic the brucellae pathway of infection and present antigens for an appropriate cytolitic T cell response. Methods E. coli was modified to express invasin of Yersinia and listerialysin O LLO of Listeria to impart the necessary infectivity and antigen releasing traits of the intracellular pathogen Brucella. This modified E. coli was considered our vaccine delivery system and was engineered to express Green Fluorescent Protein GFP

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