tailieunhanh - Báo cáo khoa hoc:" Human pluripotent embryonal carcinoma NTERA2 cl.D1 cells maintain their typical morphology in an angiomyogenic medium"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human pluripotent embryonal carcinoma NTERA2 cells maintain their typical morphology in an angiomyogenic medium | Journal of Negative Results in BioMedicine BioMed Central Research Human pluripotent embryonal carcinoma NTERA2 cells maintain their typical morphology in an angiomyogenic medium Pedro D Simões and Teresa Ramos Open Access Address Instituto de Tecnologia Biomédica Laboratório de Biomateriais Faculdade de Medicina Dentária da Universidade de Lisboa Cidade Universitária 1649-003 Lisbon Portugal Email Pedro D Simões - pedrodanielsim@ Teresa Ramos - teresaramos@ Corresponding author Published 18 April 2007 Received 6 November 2006 Journal of Negative Results in BioMedicine 2007 6 5 doi 1477-5751-6-5 Accepted 18 April 2007 This article is available from http content 6 1 5 2007 Simões and Ramos licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Pluripotent embryonal carcinomas are good potential models to study in vitro the mechanisms that control differentiation during embryogenesis. The NT2 D1 cell line is a well known system of ectodermal differentiation. Retinoic acid RA induces a dorsal pattern of differentiation essentially neurons and bone morphogenetic protein BMP or hexamethylenebisacetamide HMBA induces a more ventral epidermal pattern of differentiation. However whether these human cells could give rise to mesoderm derivatives as their counterpart in mouse remained elusive. We analyzed the morphological characteristics and transcriptional activation of genes pertinent in cardiac muscle and endothelium differentiation during the growth of NT2 D1 cells in an inductive angiomyogenic medium with or without Bone Morphogenetic Protein 2 BMP2 . Results Our experiments showed