tailieunhanh - Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection | Journal of Negative Results in BioMedicine BioMed Central Brief Report Open Access Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection Heather B Jaspan1 H Richard Gaumer2 and Robert F Garry 1 3 Address interdisciplinary Program in Molecular and Cellular Biology Tulane University School of Medicine New Orleans LA 70112 USA 2Department of Pathology Louisiana State University Medical Center New Orleans LA 70112 USA and 3Department of Microbiology and Immunology Tulane University School of Medicine New Orleans LA 70112 USA Email Heather B Jaspan - hjaspan@ H Richard Gaumer - hgaume@ Robert F Garry - rfgarry@ Corresponding author Published 12 March 2003 Received 25 August 2002 Journal of Negative Results in BioMedicine 2003 2 2 This article is available from http content 2 1 2 Accepted 12 March 2003 2003 Jaspan et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background A polymerase chain reaction PCR -based method for quantitating CD4 and CD8 mRNA could provide a means of assessing immune status of AIDS patients and other immunologically compromised persons without requiring large blood draws and could be exquisitely sensitive. Such a method would also be useful in assessing the immune status of patients retrospectively. Results Quantitative competitive reverse transcription PCR QC-RT-PCR assays were developed for measurement of CD4 and CD8 mRNA. Samples were obtained from HIV-positive and negative patients whose CD4 and CD8 counts had been determined via Flow Cytometry. The quantity of CD4 n 13 and CD8 n 28 mRNA standardized according to GAPDH mRNA quantities all determined by QC-RT-PCR were compared to cell number as .