tailieunhanh - Báo cáo khoa học: "Morphological and functional alterations in adult boar epididymis: Effects of prenatal and postnatal administration of flutamide"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Morphological and functional alterations in adult boar epididymis: Effects of prenatal and postnatal administration of flutamide. | Lydka et al. Acta Veterinaria Scandinavica 2011 53 12 http content 53 1 12 AVS ACTAVETERINARIA Scatand eaaria SCANDINAVICA RESEARCH Open Access Morphological and functional alterations in adult boar epididymis Effects of prenatal and postnatal administration of flutamide Marta Lydka Ilona Kopera-Sobota Malgorzata Kotula-Balak Katarzyna Chojnacka Dorota Zak Barbara Bilinska Abstract Background The dynamic cross-talk between epididymal cells is hormonally regulated and in part through direct cell-to-cell interactions. To date no information is available regarding possible impact of anti-androgens on the proteins involved in the gap junctional communication within the boar epididymis. Thus a question arised whether prenatal or postnatal exposure to an anti-androgen flutamide alters the expression of gap junction protein - connexin43 Cx43 and androgen receptor AR expression in the caput corpus and cauda epididymis and leads to delayed effects on morphology and function of adult pig epididymis. Methods First two experimental groups received flutamide prenatally on gestational days 20-28 and 80-88 GD20 and GD80 and further two groups were exposed to flutamide postanatally on days 2-10 and 90-98 after birth PD2 and PD90 . Epididymides were collected from adult boars. Routine histology was performed using hematoxylin-eosin staining. The expression of Cx43 and AR were analyzed using immunohistochemistry and Western blotting. Both analyses were supported by quantitative approaches to demonstrate the variations of the expression levels following the treatment. Apoptotic cells were identified using TUNEL assay. Results Histological examination revealed differences in epididymal morphology of flutamide-exposed boars when compared to controls. Scarce spermatic content were seen within the corpus and cauda lumina of GD20 PD2 and PD90 groups. Concomitantly frequency of epididymal cell apoptosis was significantly higher p after exposure to flutamide at .

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