tailieunhanh - Báo cáo y học: "Measurement of endotoxin activity in critically ill patients using whole blood neutrophil dependent chemiluminescence"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Measurement of endotoxin activity in critically ill patients using whole blood neutrophil dependent chemiluminescence. | Critical Care August 2002 Vol 6 No 4 Marshall et al. Research Measurement of endotoxin activity in critically ill patients using whole blood neutrophil dependent chemiluminescence John C Marshall1 Paul M Walker2 Debra M Foster3 David Harris4 Melanie Ribeiro4 Jeff Paice5 Alexander D Romaschin6 and Anastasia N Derzko7 1 Director of Research Medical Surgical Intensive Care Unit University Health Network Toronto Ontario Canada 2President and Chief Executive Officer Spectral Diagnostics and Sepsis Inc Toronto Ontario Canada 3Director Clinical Trials Spectral Diagnostics and Sepsis Inc Toronto Ontario Canada 4Research Associate Spectral Diagnostics and Sepsis Inc Toronto Ontario Canada 5Research Scientist Spectral Diagnostics and Sepsis Inc Toronto Ontario Canada 6Associate Professor Department of Pathobiology and Laboratory Medicine University Health Network Toronto Ontario Canada 7Data manager Spectral Diagnostics and Sepsis Inc Toronto Ontario Canada Correspondence Dr John Marshall Received 21 February 2002 Accepted 22 February 2002 Published 2 May 2002 Critical Care 2002 6 342-348 This article is online at http content 6 4 342 2002 Marshall et al. licensee BioMed Central Ltd Print ISSN 1364-8535 Online ISSN 1466-609X Abstract Background Lipopolysaccharide endotoxin from the cell wall of Gram-negative bacteria is a potent trigger for the release of host-derived inflammatory mediators. The relationship between endotoxaemia Gram-negative infection and the clinical syndrome of sepsis has been difficult to establish in part because of the limitations of available endotoxin assays. Methods We performed an observational cohort study in critically ill patients in the medical surgical intensive care unit ICU of a tertiary care hospital. Whole blood endotoxin levels on the day of ICU admission were measured using a novel chemiluminescent assay - the endotoxin activity assay EAA -and the chromogenic modification of the limulus amoebocyte .