tailieunhanh - Báo cáo thú y: " Comparison of Variable-Number Tandem-Repeat Markers typing and IS1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Comparison of Variable-Number Tandem-Repeat Markers typing and IS1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins. | Tirkkonen et al. Acta Veterinaria Scandinavica 2010 52 21 http content 52 1 21 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Comparison of Variable-Number Tandem-Repeat Markers typing and IS 1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins Taneli Tirkkonen1 2 Jaakko Pakarinen3 Elina Rintala3 Terhi Ali-Vehmas4 Harri Marttila5 Olli AT Peltoniemi1 Johanna Makinen5 6 Abstract Background Animal mycobacterioses are regarded as a potential zoonotic risk and cause economical losses world wide. M. avium subsp. hominissuis is a slow-growing subspecies found in mycobacterial infected humans and pigs and therefore rapid and discriminatory typing methods are needed for epidemiological studies. The genetic similarity of M. avium subsp. hominissuis from human and porcine origins using two different typing methods have not been studied earlier. The objective of this study was to compare the IS1245 RFLP pattern and MIRU-VNTR typing to study the genetic relatedness of M. avium strains isolated from slaughter pigs and humans in Finland with regard to public health aspects. Methods A novel PCR-based genotyping method variable number tandem repeat VNTR typing of eight mycobacterial interspersed repetitive units MIRUs was evaluated for its ability to characterize Finnish Mycobacterium avium subsp. hominissuis strains isolated from pigs n 16 and humans n 13 and the results were compared with those obtained by the conventional IS 1245 RFLP method. Results The MIRU-VNTR results showed a discriminatory index DI of 0 92 and the IS 1245 RFLP resulted in DI 0 98. The combined DI for both methods was 0 98. The MIRU-VNTR test has the advantages of being simple reproducible non-subjective which makes it suitable for large-scale screening of M. avium strains. Conclusions Both typing methods demonstrated a high degree of similarity between the strains of human and porcine origin. The .

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