tailieunhanh - Báo cáo thú y: "Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection. | Boutrup et al. Acta Veterinaria Scandinavica 2010 52 17 http content 52 1 17 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection Torsten S Boutrup1 2 Kirsten Schauser3 Jorgen S Agerholm2 Tim K Jensen1 Abstract Background Porcine proliferative enteropathy in pigs is caused by the obligate intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation PI . However knowledge of the initial in vivo interaction between porcine intestinal epithelium and the bacterium is limited. The aims of the present study were to evaluate the usefulness of a ligated small intestinal loop model to study L. intracellularis infections and to obtain information on the very early L. intracellularis-enterocyte interactions. Methods A ligated small intestinal loop model using three different L. intracellularis inocula was applied to 10-11-week-old pigs. The inocula were 1 wild type bacteria derived from overnight incubation of L. intracellularis bacteria from spontaneous disease 2 crude vaccine bacteria Enterisol Ileitis Vet and 3 vaccine bacteria propagated in cell culture. The bacteria-enterocyte interaction was visualised using immunohistochemistry on specimens derived 1 3 and 6 h PI respectively. Results Although at a low level close contact between bacteria and the enterocyte brush border including intracellular uptake of bacteria in mature enterocytes was seen at 3 and 6 h PI for the vaccine and the propagated vaccine inocula. Interaction between the wild-type bacteria and villus enterocytes was scarce and only seen at 6 h PI where a few bacteria were found in close contact with the brush border. Conclusions The ligated intestinal loop model was useful with respect to maintaining an intact intestinal morphology for up to 6 h. Furthermore the study .