tailieunhanh - Báo cáo y học: "Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples. | Alvarez-Oxiley et al. Acta Veterinaria Scandinavica 2010 52 9 http content 52 1 9 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples Andrea Vivian Alvarez-Oxiley1 Noelita Melo de Sousa1 Jean-Luc Hornick2 Kamal Touati3 Gysbert C van der Weijden4 Marcel AM Taverne4 Otto Szenci5 Jean-Frangois Beckers1 Abstract Background The involvement of placental lactogen PL in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However when circulating antibodies are present together with the hormone the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen bPL concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins IgG via a foetal catheter in vivo study . The ability of a RIA based on guinea pig anti-bPL antiserum for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins was also analyzed in in vitro and in vivo conditions. Methods Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 pg bPL mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers dilutions ranging from 1 2 500 till 1 1 280 000 . Result s Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower P in samples added with dilutions of rabbit antiserum .