tailieunhanh - Báo cáo y học: "Rhinovirus infection induces cytotoxicity and delays wound healing in bronchial epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Rhinovirus infection induces cytotoxicity and delays wound healing in bronchial epithelial cells. | Respiratory Research BioMed Central Research Open Access Rhinovirus infection induces cytotoxicity and delays wound healing in bronchial epithelial cells Apostolos Bossios Stelios Psarras Dimitrios Gourgiotis Chrysanthi L Skevaki Andreas G Constantopoulos Photini Saxoni- Papageorgiou and Nikolaos G Papadopoulos Address Allergy Department 2nd Pediatric Clinic University of Athens Athens Greece Email Apostolos Bossios - Stelios Psarras - spsarras@ Dimitrios Gourgiotis - dgourg@ Chrysanthi LSkevaki - cskevaki@ Andreas G Constantopoulos - ankonsta@ Photini Saxoni- Papageorgiou - phspapa@ Nikolaos G Papadopoulos - ngp@ Corresponding author Published 10 October 2005 Received 07 May 2005 Respiratory Research 2005 6 114 doi 1465-9921-6-114 Accepted 10 October 2005 This article is available from http content 6 1 1 14 2005 Bossios et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human rhinoviruses RV the most common triggers of acute asthma exacerbations are considered not cytotoxic to the bronchial epithelium. Recent observations however have questioned this knowledge. The aim of this study was to evaluate the ability of RV to induce epithelial cytotoxicity and affect epithelial repair in-vitro. Methods Monolayers of BEAS-2B bronchial epithelial cells seeded at different densities were exposed to RV serotypes 1b 5 7 9 14 16. Cytotoxicity was assessed chromatometrically. Epithelial monolayers were mechanically wounded exposed or not to RV and the repopulation of the damaged area was assessed by image analysis. Finally epithelial cell proliferation was assessed by quantitation of .