tailieunhanh - Báo cáo y học: "Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls. | Karlsson et al. Acta Veterinaria Scandinavica 2010 52 2 http content 52 1 2 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls Ann-Charlotte Karlsson1 2 Stefan Alenius1 Camilla Bjorkman1 Ylva Persson3 Stina Englund4 Abstract Background Reproductive disorders associated with chlamydial infection have been reported worldwide in cattle and there are indications of potential venereal transmission. Methods Semen samples from 21 dairy bulls and cauda epididymidis tissue samples from 43 beef bulls were analysed for chlamydial agent by real-time polymerase chain reaction PCR including an internal amplification control mimic . Additionally presence of antibodies against Chlamydophila Cp. abortus among the bulls was investigated with the commercial Pourquier ELISA Cp. abortus serum verification kit. Results No chlamydial agent was detected by PCR in either the semen samples or in the tissue samples. Additionally no antibodies against Cp. abortus were detected. Conclusions The results suggest that Cp. abortus is very rare or absent in Swedish bulls and thus the risk for venereal transmission of chlamydial infection through their semen is low. However because Chlamydophila spp. infection rates seem to differ throughout the world it is essential to clarify the relative importance of transmission of the infection through semen on cattle fertility. Background Bovine chlamydiosis has been associated with several disease manifestations 1 . Reproductive disorders such as sporadic abortions and reduced fertility linked with chlamydial infection have been reported from Germany 2 3 Great Britain 4 Italy 5 Japan 6 Switzerland 7 Taiwan 8 and the USA 9 . In Sweden the incidence of abortion in cows is low. However reproductive disorders and infertility are major causes of culling but are often difficult to be diagnosed. Chlamydial infection in