tailieunhanh - Báo cáo y học: "Anaplasma phagocytophilum in Danish sheep: confirmation by DNA sequencing"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Anaplasma phagocytophilum in Danish sheep: confirmation by DNA sequencing. | Acta Veterinaria Scandinavica BioMed Central Brief communication Anaplasma phagocytophilum in Danish sheep confirmation by DNA sequencing Anne M Kiilerich Henrik Christensen and Stig M Thamsborg Address Department of Veterinary Disease Biology Faculty of Life Sciences University of Copenhagen Dyrl gevej 88 DK-1870 Frederiksberg C Denmark Email Anne M Kiilerich - amkiilerich@ Henrik Christensen - hech@ Stig M Thamsborg - smt@ Corresponding author Open Access Published 21 December 2009 Received 15 May 2009 . . J. . __ rr Accepted 21 December 2009 Acta Veterinaria Scandinavica 2009 51 55 doi 86 1751-0147-51 -55 This article is available from http content 51 1 55 2009 Kiilerich et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background The presence of Anaplasma phagocytophilum an Ixodes ricinus transmitted bacterium was investigated in two flocks of Danish grazing lambs. Direct PCR detection was performed on DNA extracted from blood and serum with subsequent confirmation by DNA sequencing. Methods 3l samples obtained from clinically normal lambs in 2000 from Fussing0 Jutland and 12 samples from ten lambs and two ewes from a clinical outbreak at Feddet Zealand in 2006 were included in the study. Some of the animals from Feddet had shown clinical signs of polyarthritis and general unthriftiness prior to sampling. DNA extraction was optimized from blood and serum and detection achieved by a 16S rRNA targeted PCR with verification of the product by DNA sequencing. Results Five DNA extracts were found positive by PCR including two samples from 2000 and three from 2006. For both .