tailieunhanh - Báo cáo y học: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells. | Respiratory Research BioMed Central Research Open Access Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4 cells Carlo Vancheri Claudio Mastruzzo Elisa Trovato-Salinaro Elisa Gili Debora Lo Furno Maria P Pistorio Massimo Caruso Cristina La Rosa Claudia Crimi Marco Failla and Nunzio Crimi Address Department of Internal and Specialistic Medicine Section of Respiratory Medicine University of Catania Catania 95125 Italy Email Carlo Vancheri - vancheri@ Claudio Mastruzzo - mastruzzo@ Elisa Trovato-Salinaro - elisatrovato@ Elisa Gili - elisagili@ Debora Lo Furno - Maria P Pistorio - azne_679@ Massimo Caruso - azne_679@ Cristina La Rosa - engypsy@ Claudia Crimi - crimi@ Marco Failla - vancheri@ Nunzio Crimi - crimi@ Corresponding author Published 13 September 2005 Received 01 June 2005 Respiratory Research 2005 6 103 doi 1465-9921-6-103 Accepted 13 September 2005 This article is available from http content 6 1 103 2005 Vancheri et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background T lymphocytes are demonstrated to play an important role in several chronic pulmonary inflammatory diseases. In this study we provide evidence that human lung fibroblasts are capable of mutually interacting with T-lymphocytes leading to functionally significant responses by T-cells and fibroblasts. Methods Human lung fibroblast were co-cultured with PMA-ionomycin activated T-CD4 lymphocytes for 36 hours. Surface as well as intracellular proteins expression relevant to fibroblasts and lymphocytes activation were evaluated by means of flow .