tailieunhanh - Báo cáo khoa học: " Multi-locus sequence typing of Salmonella enterica subsp. enterica"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Multi-locus sequence typing of Salmonella enterica subsp. enterica . | Noda et al. Acta Veterinaria Scandinavica 2011 53 38 http content 53 1 38 AVS ACTAVETERINARIA SCANDINAVICA BRIEF COMMUNICATION Open Access Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004 1 2 1 3 3 1 Tamie Noda Koichi Murakami Tetsuo Asai Yoshiki Etoh Tomoe Ishihara Toshiro Kuroki Kazumi Horikawa and Shuji Fujimoto4 Abstract Salmonella enterica subsp. enterica serovar Enteritidis S. Enteritidis was responsible for a worldwide pandemic during the 1980s and 1990s however changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing MLST . Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004 consisting of 27 human strains from individual episodes a bovine strain a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation it is likely to be a stable lineage derived from an ancestor before the 1980s and or 1990s pandemic in Japan. Findings Salmonella enterica subsp. enterica serovar Enteritidis S. Enteritidis was responsible for a worldwide pandemic during the 1980s and 1990s however changes in the dominant lineage before and after this event remain unknown. It is also difficult to explain why multiple clones of S. Enteritidis defined by phage types PTs infecting chicken reproductive tissues emerged simultaneously in geographically separate countries during this .