tailieunhanh - Báo cáo khoa học: "Changes in Selected Biochemical Indices Resulting from Various Pre-sampling Handling Techniques in Broilers"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Changes in Selected Biochemical Indices Resulting from Various Pre-sampling Handling Techniques in Broilers. | Chloupek et al. Acta Veterinaria Scandinavica 2011 53 31 http content 53 1 31 AVS ACTAVETERINARIA tTnTXZ 0 SCANDINAVICA RESEARCH Open Access Changes in Selected Biochemical Indices Resulting from Various Pre-sampling Handling Techniques in Broilers 1 1 2 1 Petr Chloupek Iveta Bedanova Jan Chloupek and Vladimir Vecerek Abstract Background Since it is not yet clear whether it is possible to satisfactorily avoid sampling-induced stress interference in poultry more studies on the pattern of physiological response and detailed quantification of stress connected with the first few minutes of capture and pre-sampling handling in poultry are required. This study focused on detection of changes in the corticosterone level and concentrations of other selected biochemical parameters in broilers handled in two different manners during blood sampling involving catching carrying restraint and blood collection itself that lasted for various time periods within the interval 30-180 seconds. Methods Stress effects of pre-sampling handling were studied in a group n 144 of unsexed ROSS 308 broiler chickens aged 42 d. Handling catching carrying restraint and blood sampling itself was carried out in a gentle caught held and carried carefully in an upright position or rough caught by the leg held and carried with lack of care in inverted position manner and lasted for 30 s 60 s 90 s 120 s 150 s and 180 s. Plasma corticosterone albumin glucose cholesterol lactate triglycerides and total protein were measured in order to assess the stress-induced changes to these biochemical indices following handling in the first few minutes of capture. Results Pre-sampling handling in a rough manner resulted in considerably higher plasma concentrations of all biochemical indices monitored when compared with gentle handling. Concentrations of plasma corticosterone after 150 and 180 s of handling were considerably higher P than concentrations after 30-120 s of handling regardless