tailieunhanh - Báo cáo y học: "Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells. | Respiratory Research BioMed Central Research Open Access Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells Steven Bourke 1 Helen S Mason1 Zea Borok2 Kwang-Jin Kim2 Edward D Crandall2 and Paul J Kemp 1 2 Address 1Cardiff School of Biosciences Museum Avenue Cardiff CF10 3US Wales UK and 2Will Rogers Institute Pulmonary Research Center Division of Pulmonary and Critical Care Medicine Keck School of Medicine Los Angeles CA 90033 USA Email Steven Bourke - bourkesa@ Helen S Mason - masonhs@ Zea Borok - zborok@ Kwang-Jin Kim - kjkim@ Edward D Crandall - ecrandal@ Paul J Kemp - kemp@ Corresponding author Published 27 April 2005 Received 21 December 2004 Respiratory Research 2005 6 40 doi 1465-9921 -6-40 Accepted 27 April 2005 This article is available from http content 6 1 40 2005 Bourke et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the alveolar type I cell might significantly contribute to the overall ion and fluid homeostasis of the lung direct assessment of functional ion channels in type I cells has remained elusive. Methods Here we describe a development of a lung slice preparation that has allowed positive identification of alveolar type I cells within an intact and viable alveolar epithelium using living cell immunohistochemistry. Results This technique has