tailieunhanh - Báo cáo khoa học: "Effects of crp deletion in Salmonella enterica serotype Gallinarum"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Effects of crp deletion in Salmonella enterica serotype Gallinarum. | Acta Veterinaria Scandinavica BioMed Central Research Effects of crp deletion in Salmonella enterica serotype Gallinarum Valentina Rosu1 Mark S Chadfield2 Antonella Santona1 Jens P Christensen2 Line E Thomsen2 Salvatore Rubino1 and John E Olsen 2 Open Access Address Department of Biomedical Science University of Sassari Viale San Pietro 43B 07100 Sassari Italy and 2Department of Veterinary Pathobiology Faculty of Life Sciences University of Copenhagen Stigbojlen 4 DK-1870 Frederiksberg C Denmark Email Valentina Rosu - v_rosu@ Mark S Chadfield - msc@ Antonella Santona - santona@ Jens P Christensen - jpc@ Line E Thomsen - let@ Salvatore Rubino - rubino@ John E Olsen - jeo@ Corresponding author Published 8 May 2007 Received 27 December 2006 Acta Veterinaria Scandinavian 2007 49 14 doi 1751-0147-49-14 Accepted 8 May 2007 This article is available from http content 49 1 14 2007 Rosu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Salmonella enterica serotype Gallinarum S. Gallinarum remains an important pathogen of poultry especially in developing countries. There is a need to develop effective and safe vaccines. In the current study the effect of crp deletion was investigated with respect to virulence and biochemical properties and the possible use of a deletion mutant as vaccine candidate was preliminarily tested. Methods Mutants were constructed in S. Gallinarum by P22 transduction from Salmonella Typhimurium S. Typhimurium with deletion of the crp gene. The effect was characterized by measuring biochemical properties and by testing of invasion in a chicken loop model and by challenge of six-day-old .