tailieunhanh - Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle. | Acta Veterinaria Scandinavica BioMed Central Research Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle Marit Pringle 1 Christer Bergsten2 Lise-Lotte Fernstrom1 Helena Hook1 and Karl-Erik Johansson1 Open Access Address Dept of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Box 7009 SE-75007 Uppsala Sweden and 2Dept of Animal Environment and Health Swedish University of Agricultural Sciences Box 234 SE-53223 Skara Sweden Email Marit Pringle - Christer Bergsten - Lise-Lotte Fernstrom - Helena Hook - Karl-Erik Johansson - Corresponding author Published 20 October 2008 Received I July 2008 Acta Veterinaria Scandinavica 2008 50 40 doi 1751-0147-50-40 Accepted 20 October 2008 This article is available from http content 50 1 40 2008 Pringle et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Digital dermatitis in cattle is an emerging infectious disease. Ulcerative lesions are typically located on the plantar skin between the heel bulbs and adjacent to the coronet. Spirochetes of the genus Treponema are found in high numbers in the lesions and are likely to be involved in the pathogenesis. The aim of this study was to obtain pure cultures of spirochetes from cattle with digital dermatitis and to describe them further. Methods Tissue samples and swabs from active digital dermatitis lesions were used for culturing. Pure isolates were subjected to molecular typing through 16S rRNA gene sequencing pulsed-field gel .