tailieunhanh - Báo cáo y học: " Quantification of collagen and proteoglycan deposition in a murine model of airway remodelling"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Quantification of collagen and proteoglycan deposition in a murine model of airway remodelling. | Respiratory Research BioMed Central Research Open Access Quantification of collagen and proteoglycan deposition in a murine model of airway remodelling Alistair K Reinhardt Stephen E Bottoms Geoffrey J Laurent and Robin J McAnulty Address Centre for Respiratory Research University College London Rayne Building 5 University Street London WC1E 6JJ UK Email Alistair K Reinhardt - Stephen E Bottoms - Geoffrey J Laurent - Robin J McAnulty - Corresponding author Published 08 April 2005 Received 27 September 2004 Respiratory Research 2005 6 30 doi 1465-9921 -6-30 Accepted 08 April 2005 This article is available from http content 6 1 30 2005 Reinhardt et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Sub-epithelial extracellular matrix deposition is a feature of asthmatic airway remodelling associated with severity of disease decline in lung function and airway hyperresponsiveness. The composition of and mechanisms leading to this increase in subepithelial matrix and its importance in the pathogenesis of asthma are unclear. This is partly due to limitations of the current models and techniques to assess airway remodelling. Methods In this study we used a modified murine model of ovalbumin sensitisation and challenge to reproduce features of airway remodelling including a sustained increase in sub-epithelial matrix deposition. In addition we have established techniques to accurately and specifically measure changes in sub-epithelial matrix deposition using histochemical and immunohistochemical staining in conjunction with digital image analysis and applied these to the measurement of collagen and .