tailieunhanh - Báo cáo y học: " Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts. | Respiratory Research BioMed Central Research Open Access Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts Diane L Carlisle1 2 Toni M Hopkins1 Autumn Gaither-Davis1 Michele J Silhanek1 James D Luketich2 Neil A Christie2 and Jill M Siegfried 1 2 Address Department of Pharmacology University of Pittsburgh Pittsburgh PA USA and 2University of Pittsburgh and Lung and Thoracic Malignancies Program University of Pittsburgh Cancer Institute Pittsburgh PA USA Email Diane L Carlisle - dlc4@ Toni M Hopkins - toh11@ Autumn Gaither-Davis - agaither@ Michele J Silhanek - msilhanek2978@ James D Luketich - luketichjd@ Neil A Christie - Christiena@ Jill M Siegfried - siegfriedjm@ Corresponding author Published 10 December 2004 Received 27 August 2004 Accepted 10 December 2004 Respiratory Research 2004 5 27 doi 1465-9921 -5-27 This article is available from http content 5 1 27 2004 Carlisle et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Non-neuronal cells including those derived from lung are reported to express nicotinic acetylcholine receptors nAChR . We examined nAChR subunit expression in short-term cultures of human airway cells derived from a series of never smokers ex-smokers and active smokers. Methods and Results At the mRNA level human bronchial epithelial HBE cells and airway fibroblasts expressed a range of nAChR subunits. In multiple cultures of both cell types mRNA was detected for subunits that constitute functional muscle-type and neuronal-type pentomeric receptors. Two immortalized cell lines derived from HBE .