tailieunhanh - Báo cáo y học: " The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose. | Respiratory Research BioMed Central Research Open Access The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose Graeme R Zosky 1 2 Christophe von Garnier1 2 Philip A Stumbles1 2 Patrick G Holt1 2 Peter D Sly1 2 and Debra J Turner1 2 Address Telethon Institute for Child Health Research West Perth 6872 Australia and 2Centre for Child Health Research University of Western Australia Crawley 6009 Australia Email Graeme R Zosky - graemez@ Christophe von Garnier - christophe@ Philip A Stumbles - phils@ Patrick G Holt - patrick@ Peter D Sly - peters@ Debra J Turner - debrat@ Corresponding author Published 23 September 2004 Received 02 July 2004 Accepted 23 September 2004 Respiratory Research 2004 5 15 doi 1465-9921-5-15 This article is available from http content 5 1 15 2004 Zosky et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Considerable variation exists in the protocols used to induce hyperresponsiveness in murine models of allergic sensitisation. We examined the effect of varying the number of antigen exposures at challenge on the development of methacholine responsiveness in systemically sensitised mice. Methods BALB c mice were sensitised with ovalbumin OVA challenged with 1 3 or 6 OVA aerosols. Lung function was measured using low frequency forced oscillations and partitioned into components representing the airways Raw and lung parenchyma tissue damping G and tissue elastance H . Responsiveness to inhaled methacholine MCh inflammatory cell profile and circulating IgE were assessed 24 and 48 hours after challenge. The threshold dose of MCh .