tailieunhanh - Báo cáo y học: "A mechanism of airway injury in an epithelial model of mucociliary clearance"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"A mechanism of airway injury in an epithelial model of mucociliary clearance. | Respiratory Research BioMed Central Research Open Access A mechanism of airway injury in an epithelial model of mucociliary clearance Darryl W O Brien Melanie I Morris Jie Ding J Gustavo Zayas ShushengTai and Malcolm King Address Pulmonary Research Group Department of Medicine University of Alberta Edmonton Alberta Canada Email Darryl W O Brien - do1@ Melanie I Morris - mimorris@ Jie Ding - jie0609@ J Gustavo Zayas - ShushengTai - stai@ Malcolm King - Corresponding author Published 24 August 2004 Received 13 May 2004 Respiratory Research 2004 5 10 doi l465-992l-5-l0 Accepted 24 August 2004 This article is available from http content 5 l l0 2004 O Brien et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We studied the action of sodium metabisulphite on mucociliary transport in a frog palate epithelial injury model hypothesizing that it may be useful for the study of mechanisms of airway injury. Sodium metabisulphite MB releases SO2 on contact with water. SO2 is a pollutant in automobile fumes and may play a role in the exacerbation of airway disease symptoms. We first investigated its effect on mucociliary clearance. MB 10-1 M increased mucociliary clearance time MCT by of control values p n 7 . MB l0-4 and 10-2 M did not interfere with mucus clearance time compared to control values. In MB-treated frog palates MCT did not return to control values after one hour control vs. MB p n 7 . Scanning EM images of epithelial tissue were morphometrically analyzed and showed a 25 l2 loss of ciliated cells in MB palates compared to controls with an intact .