tailieunhanh - Báo cáo y học: " Activation of macrophage nuclear factor-κB and induct ion of inducible nitric oxide synthase by LPS"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Activation of macrophage nuclear factor-κB and induct ion of inducible nitric oxide synthase by LPS. | Available online http content 3 1 23 Research article Activation of macrophage nuclear factor-KBand induct ion of inducible nitric oxide synthase by LPS Ying-Hua Li1 2 Zhong-Qun Yan3 Annelie Brauner2 and Kjell Tullus1 1Astrid Lindgren Children s Hospital Karolinska Institutet Stockholm Sweden 2Department of Clinical Microbiology Karolinska Hospital Stockholm Sweden 3Center for Molecular Medicine Karolinska Hospital Stockholm Sweden Correspondence Ying-Hua Li - Received 22 November 2001 Revisions requested 26 February 2002 Revisions received 4 April 2002 Accepted 6 June 2002 Published 5 September 2002 Respir Res 2002 3 23 2002 Tullus et al. licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background Chronic lung disease CLD of prematurity is a major problem of neonatal care. Bacterial infection and inflammatory response have been thought to play an important role in the development of CLD and steroids have been given with some benefit to neonates with this disease. In the present study we assessed the ability of lipopolysaccharide LPS to stimulate rat alveolar macrophages to produce nitric oxide NO express inducible nitric oxide synthase iNOS and activate nuclear factor-kB NF-kB in vitro. In addition we investigated the impact of dexamethasone and budesonide on these processes. Methods Griess reaction was used to measure the nitrite level. Western blot and a semi-quantitative RT-PCR were performed to detect iNOS expression. Electrophoretic mobility shift assay EMSA was performed to analyze the activation of NF-kB. Results We found that LPS stimulated the rat alveolar macrophages to produce NO in a dose 10 ng ml and time dependent manner p . This effect was further enhanced by IFN-Y 10 lU ml p but was attenuated by budesonide 1 0-4-10-10 M and dexamethasone 1 0-4-10-6 M p . The mRNA and protein levels of iNOS were also induced in response to LPS and attenuated by steroids. .

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