tailieunhanh - Báo cáo khoa hoc:" Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1 | Souza et al. Journal of Negative Results in BioMedicine 2010 9 4 http content 9 1 4 RESEARCH JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE Open Access Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1 Bruno R Souza 1 Karen CL Torres1 Débora M Miranda1 Bernardo S Motta1 Estêvão Scotti-Muzzi1 Melissa M Guimarães1 Daniel S Carneiro1 Daniela VF Rosa1 Renan P Souza1 Helton J Reis2 Andreas Jeromin3 and Marco A Romano-Silva 1 Abstract Background Schizophrenia is the major psychiatry disorder which the exact cause remains unknown. However it is well known that dopamine-mediated neurotransmission imbalance is associated with this pathology and the main target of antipsychotics is the dopamine receptor D2. Recently it was described alteration in levels of two dopamine signaling related proteins in schizophrenic prefrontal cortex PFC Neuronal Calcium Sensor-1 NCS-1 and DARPP-32. NCS-1 which is upregulated in PFC of schizophrenics inhibits D2 internalization. DARPP-32 which is decreased in PFC of schizophrenics is a key downstream effector in transducing dopamine signaling. We previously demonstrated that antipsychotics do not change levels of both proteins in rat s brain. However since NCS-1 and DARPP-32 levels are not altered in wild type rats we treated wild type PC12 cells PC12 WT and PC12 cells stably overexpressing NCS-1 PC12 Clone with antipsychotics to investigate if NCS-1 upregulation modulates DARPP-32 expression in response to antipsychotics treatment. Results We chronically treated both PC12 WT and PC12 Clone cells with typical Haloperidol or atypical Clozapine and Risperidone antipsychotics for 14 days. Using western blot technique we observed that there is no change in NCS-1 and DARPP-32 protein levels in both PC12 WT and PC12 Clone cells after typical and atypical antipsychotic treatments. Conclusions Because we observed no alteration in NCS-1 and DARPP-32 levels in both PC12 WT and