tailieunhanh - Báo cáo y học: " Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis | Available online http respiratory-research content 3 1 17 Research article Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis Roberto Cruz-Gervis1 3 Arlene A Stecenko1 Ryszard Dworski1 Kirk B Lane1 James E Loyd1 Richard Pierson2 Gayle King1 and Kenneth L Brigham1 1 Center for Lung Research Division of Allergy Pulmonary and Critical Care Medicine Department of Medicine Vanderbilt University Nashville Tennessee USA. 2Department of Surgery Vanderbilt University Nashville Tennessee USA. 3Department of Internal Medicine Meharry Medical College Nashville Tennessee USA. Correspondence Roberto Cruz-Gervis - Received 29 May 2001 Revisions requested 12 July 2001 Revisions received 14 November 2001 Accepted 21 December 2001 Published 25 February 2002 Respir Res 2002 3 17 2002 Cruz-Gervis et al. licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background Prostanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis IPF we hypothesized that fibroblasts HF cultured from the lungs of patients with IPF HF-IPF have an altered balance between profibrotic thromboxane TX A2 and antifibrotic prostacyclin PGI2 prostaglandins PGs when compared with normal human lung fibroblasts HF-Nl . Methods We measured inducible cyclooxygenase COX -2 gene and protein expression and a profile of prostanoids at baseline and after IL-1 p stimulation. Results In both HF-IPF and HF-NL Cox-2 expression was undetectable at baseline but was significantly upregulated by IL-1p. PGE2 was the predominant COX product in IL-1p-stimulated cells with no significant difference between HF-IPF and HF-NL vs. ng 106 cells 30 min respectively P . TXB2 the stable metabolite of TXA2 production was .

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