tailieunhanh - Báo cáo y học: " Private specificities can dominate the humoral response to self-antigens in patients with cryptogenic fibrosing alveolitis "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:: " Private specificities can dominate the humoral response to self-antigens in patients with cryptogenic fibrosing alveolitis. | Available online http content 2 2 119 Primary research Private specificities can dominate the humoral response to self-antigens in patients with cryptogenic fibrosing alveolitis Cleo Robinson Marinella Callow Sandra Stevenson Bruce WS Robinson and Richard A Lake University Department of Medicine Western Australian Institute for Medical Research Queen Elizabeth II Medical Centre Perth Western Australia Correspondence Dr RA Lake University Department of Medicine Queen Elizabeth II Medical Centre 4th Floor G Block Nedlands Perth Western Australia 6009. Tel 618 9346 3127 fax 618 9346 2816 e-mail rlake@ Received 24 November 2000 Revisions requested 12 January 2001 Revisions received 29 January 2001 Accepted 31 January 2001 Published 20 February 2000 Respir Res 2001 2 119-124 2001 Robinson et al licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background The pathogenetic mechanisms that underlie the interstitial lung disease cryptogenic fibrosing alveolitis CFA may involve an immunological reaction to unidentified antigens in the lung resulting in tissue damage. Method In order to identify the range of target autoantigens we used expression cloning employing serum from an index patient as the probe against an expressed cDNA library that was derived from a tumour cell line. We screened over 5 X 105 recombinants and obtained sequence information on three antigens that had provoked strong responses with immunoglobulin heavy chain class switching presumably as a consequence of T-cell recognition. Results All of the antigens were identifiable by comparison with sequence data from the US National Center for Biotechnology Information. Alanyl tRNA synthetase ATS was picked on six occasions five of these incidences reflected independent recombination events indicating that the library was not biased. Antibodies to ATS anti-PL-12 represent the most common reactivity that defines the antisynthetase syndrome .

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