tailieunhanh - Báo cáo y học: " Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture. | Available online http content 2 5 295 Research article Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture Yunkui Zhu C Magnus Skold Xiangde Liu Hangjun Wang Tadashi Kohyama Fu-Qiang Wen Ronald F Ertl and Stephen I Rennard University of Nebraska Medical Center Omaha Nebraska USA Karolinska Hospital Stockholm Sweden Mount Sinai Hospital Pathology and Laboratory Medicine Toronto Ontario Canada Correspondence Stephen I Rennard MD University of Nebraska Medical Center 985125 Nebraska Medical Center Omaha Nebraska 68198-5125 USA. Tel 1 402 559 7313 fax 1 402 559 4878 e-mail srennard@ Received 5 March 2001 Revisions requested 14 May 2001 Revisions received 13 July 2001 Accepted 8 August 2001 Published 4 September 2001 Respir Res 2001 2 295-299 2001 Zhu et al licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in threedimensional collagen gels. Methods Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Results Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes P . However with extended co-culture contraction of the collagen gels was greatly augmented P . In addition with extended co-culture degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation P . Prostaglandin E2 production was significantly increased by co-culture and its presence attenuated collagen degradation. Conclusion The current study therefore demonstrates that interaction between