tailieunhanh - Báo cáo y học: "High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts. | Wahamaa et al. Arthritis Research Therapy 2011 13 R136 http content 13 4 R136 RESEARCH ARTICLE Open Access High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts 1 1 2 1 1 Heidi Wahamaa Hanna Schierbeck Hulda S Hreggvidsdottir Karin Palmblad Anne-Charlotte Aveberger Ulf Andersson1 and Helena Erlandsson Harris2 Abstract Introduction In addition to its direct proinflammatory activity extracellular high mobility group box protein 1 HMGB1 can strongly enhance the cytokine response evoked by other proinflammatory molecules such as lipopolysaccharide LPS CpG-DNA and IL-1P through the formation of complexes. Extracellular HMGB1 is abundant in arthritic joint tissue where it is suggested to promote inflammation as intra-articular injections of HMGB1 induce synovitis in mice and HMGB1 neutralizing therapy suppresses development of experimental arthritis. The aim of this study was to determine whether HMGB1 in complex with LPS interleukin IL -1a or IL-1P has enhancing effects on the production of proinflammatory mediators by rheumatoid arthritis synovial fibroblasts RASF and osteoarthritis synovial fibroblasts OASF . Furthermore we examined the toll-like receptor TLR 4 and IL-1RI requirement for the cytokine-enhancing effects of the investigated HMGB1-ligand complexes. Methods Synovial fibroblasts obtained from rheumatoid arthritis RA and osteoarthritis OA patients were stimulated with HMGB1 alone or in complex with LPS IL-1a or IL-1p. Tumour necrosis factor TNF production was determined by enzyme-linked immunospot assay ELISPOT assessment. Levels of IL-10 IL-1-p IL-6 and IL-8 were measured using Cytokine Bead Array and matrix metalloproteinase MMP 3 production was determined by ELISA. Results Stimulation with HMGB1 in complex with LPS IL-1a or IL-1P enhanced production of TNF IL-6 and IL-8. HMGB1 in complex with IL-1P increased MMP production from both RASF and