tailieunhanh - Báo cáo y học: "Modulation of interleukin-1b-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Modulation of interleukin-1b-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts. | Turner-Brannen et al. Arthritis Research Therapy 2011 13 R129 http content 13 4 R129 RESEARCH ARTICLE Open Access Modulation of interleukin-1 p-induced inflammatory responses by a synthetic cationic innate defence regulator peptide IDR-1002 in synovial fibroblasts 1 1 1 3 Emily Turner-Brannen Ka-Yee Choi Dustin ND Lippert John P Cortens Robert EW Hancock Hani El-Gabalawy1 and Neeloffer Mookherjee1 2 Abstract Introduction Innate defence regulator IDR peptides are synthetic cationic peptides variants of naturally occurring innate immune effector molecules known as host defence peptides. IDR peptides were recently demonstrated to limit infection-associated inflammation selectively without compromising host innate immune functions. This study examined the impact of a 12-amino acid IDR peptide IDR-1002 in pro-inflammatory cytokine interleukin IL -1p-induced responses in synovial fibroblasts a critical cell type in the pathogenesis of inflammatory arthritis. Methods Human fibroblast-like synoviocytes FLS were stimulated with IL-1p in the presence and absence of IDR-1002. Production of enzyme matrix metalloproteinase-3 MMP-3 and IL-1-receptor antagonist IL-1RA was monitored by enzyme-linked immunosorbent assay ELISA and various chemokines were evaluated by using multiplex cytometric bead array. Transcriptional responses were analyzed by quantitative real-time PCR. The impact on IL-1p-induced proteome was investigated by quantitative proteomics by using isobaric tags. IL-1p-induced pathways altered by IDR-1002 implicated by the proteomics analyses were further investigated by using various immunochemical assays. Cellular uptake of the peptide was monitored by using a biotinylated IDR-1002 peptide followed by microscopy probing with streptavidin-Alexa Fluor. Results This study demonstrated that IDR-1002 suppressed the production of IL-1p-induced MMP-3 and monocyte chemotactic protein-1 MCP-1 in contrast IDR-1002 enhanced the production of IL-1RA .