tailieunhanh - Báo cáo khoa hoc:" Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro | Journal of Negative Results in BioMedicine Research Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro Svetlana Mastitskaya and Bernd Denecke BioMed Central Open Access Address Interdisciplinary Centre for Clinical Research IZKF BIOMAT. RWTH Aachen University Aachen Germany Email Svetlana Mastitskaya - Bernd Denecke - Corresponding author Published 10 November 2009 Received II March 2009 . . I j-r-r r- r I n I I Accepted 10 November 2009 Journal of Negative Results in BioMedicine 2009 8 11 doi 1477-5751-8-11 This article is available from http content 8 1 1 1 2009 Mastitskaya and Denecke licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human mesenchymal stem cells hMSCs are broadly discussed as a promising cell population amongst others for regenerative therapy of ischemic heart disease and its consequences. Although cardiac-specific differentiation of hMSCs was reported in several in vitro studies these results were sometimes controversial and not reproducible. Results In our study we have analyzed different published protocols of cardiac differentiation of hMSCs and their modifications including the use of differentiation cocktails different biomaterial scaffolds co-culture techniques and two- and three-dimensional cultures. We also studied whether 5 -azacytidin and trichostatin A treatments in combination with the techniques mentioned above can increase the cardiomyogenic potential of hMSCs. We found that hMSCs failed to generate functionally active cardiomyocytes in vitro although part of the cells demonstrated increased levels of cardiac-specific gene expression when treated .