tailieunhanh - Báo cáo y học: " Multiple P2Y receptors couple to calcium-dependent, chloride channels in smooth muscle cells of the rat pulmonary artery"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Multiple P2Y receptors couple to calcium-dependent, chloride channels in smooth muscle cells of the rat pulmonary artery. | Respiratory Research BioMed Central Research Open Access Multiple P2Y receptors couple to calcium-dependent chloride channels in smooth muscle cells of the rat pulmonary artery Krongkarn Chootip1 2 Alison M Gurney1 and Charles Kennedy 1 Address Department of Physiology and Pharmacology University of Strathclyde Strathclyde Institute for Biomedical Sciences John Arbuthnott Building 27 Taylor Street Glasgow G4 ONR UK and 2Department of Physiology Faculty of Medical Science Naresuan University Phitsanulok 65000 Thailand Email Krongkarn Chootip - Alison M Gurney - Charles Kennedy - Corresponding author Published 26 October 2005 Received 20 July 2005 Respiratory Research 2005 6 124 doi 1465-9921-6-124 Accepted 26 October 2005 This article is available from http content 6 1 124 2005 Chootip et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Uridine 5 -triphosphate UTP and uridine 5 -diphosphate UDP act via P2Y receptors to evoke contraction of rat pulmonary arteries whilst adenosine 5 -triphosphate ATP acts via P2X and P2Y receptors. Pharmacological characterisation of these receptors in intact arteries is complicated by release and extracellular metabolism of nucleotides so the aim of this study was to characterise the P2Y receptors under conditions that minimise these problems. Methods The perforated-patch clamp technique was used to record the Ca2 -dependent Cl-current ICl Ca activated by P2Y receptor agonists in acutely dissociated smooth muscle cells of rat small SPA and large LPA intrapulmonary arteries held at -50 mV. Contractions to ATP were measured in isolated muscle rings. Data were .